Coding

Part:BBa_K4256007:Design

Designed by: Yuqi Liu   Group: iGEM22_UCAS-China   (2022-09-29)


ech (enoyl-CoA hydratase/aldolase) from Streptomyces sp. V-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 544
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This enzyme is involved in the second and final steps of the production of vanillin from ferulic acid. Its functions have been characterized in E. coli according to the reference.


Source

Source：Streptomyces sp. V-1 Reference: Yang,W., Tang,H., Ni,J., Wu,Q., Hua,D., Tao,F. and Xu,P. Characterization of two streptomyces enzymes that convert ferulic Acid to vanillin. PLoS ONE 8 (6), E67339 (2013)

References